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luc b16f10 cells  (Novus Biologicals)


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    Novus Biologicals luc b16f10 cells
    Luc B16f10 Cells, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luc+b16f10+cells/Exosome+Standards+(B16F10+cell+line)/pm40570715-199-6-16
    Average 93 stars, based on 1 article reviews
    luc b16f10 cells - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Nanoparticles-mediated intratumoral gene editing targeting PD-L1 and Galectin-9 for improved cancer immunotherapy.
    Article Snippet: Tumor cells from tumor tissues and blood were obtained for PD-L1 and Gal-9 disruption detections processed by Cleavage Detection Kit (Invitrogen, Cat no. A24372).

    Luciferase:

    Article Title: Nanoparticles-mediated intratumoral gene editing targeting PD-L1 and Galectin-9 for improved cancer immunotherapy.
    Article Snippet: Tumor cells from tumor tissues and blood were obtained for PD-L1 and Gal-9 disruption detections processed by Cleavage Detection Kit (Invitrogen, Cat no. A24372).

    FACS:

    Article Title: Nanoparticles-mediated intratumoral gene editing targeting PD-L1 and Galectin-9 for improved cancer immunotherapy.
    Article Snippet: Tumor cells from tumor tissues and blood were obtained for PD-L1 and Gal-9 disruption detections processed by Cleavage Detection Kit (Invitrogen, Cat no. A24372).



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    ( A and B ) CD8 + T cells isolated from 2C transgenic WT or PERK-cKO mice were labeled with CFSE and stimulated with allogeneic APCs from BDF1 mice for 4 days; levels of proinflammatory cytokines (IFN-γ, TNF-α) in CD8 + T cells were analyzed by flow cytometry ( A ). Percentages of CFSE lo IFN-γ + CD8 + and CFSE lo TNF-α + CD8 + T cells among gated H2K d– CD8 + T cells are shown ( B ). ( C and D ) CD8 + T cells isolated from 2C transgenic WT or PERK-cKO mice were labeled with CFSE and stimulated with SIY peptides (10 nM) and syngeneic B6 APCs (Ly5.1); levels of cytokines were analyzed similarly. ( E – G ) Lethally irradiated BDF1 mice (1,200 cGy) received TCD-BM cells (5 × 10 6 ) alone or along with P815-luc cells (5,000) with or without CD25-removed T cells (3 × 10 6 ) from 2C WT or PERK-cKO donors. Body weight loss ( E ) and GVHD scores ( F ) were monitored after BMT. Tumor growth in BDF1 recipients was monitored via BLI ( G ); n = 5 per BMT group. ( H ) CD8 + T cells from pMEL WT or PERK-cKO mice were stimulated with gp100 peptides and B6 APCs (Ly5.1) for 3 days; levels of IFN-γ and IL-17 in CD8 + T cells were analyzed. Percentages of IFN-γ + CD8 + and IL-17 + CD8 + T cells among gated CD45.1 – CD8 + T cells are shown. ( I and J ) Lethally irradiated BDF1 mice received TCD-BM cells (5 × 10 6 ) along with <t>B16F10-luc</t> cells with or without CD25-removed T cells (3 × 10 6 ) from pMEL WT or PERK-cKO donors; n = 3 for tumor only, n = 5–6 for WT or PERK-cKO ( I ). Survival ( J ) was monitored after BMT. Log-rank (Mantel-Cox) test ( J ) and non-parametric Mann-Whitney U test ( E and F ) were used to compare groups. Data in B , D , and H are represented as mean ± SD with biological replicates; significance was determined using a 2-tailed unpaired Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    ( A and B ) CD8 + T cells isolated from 2C transgenic WT or PERK-cKO mice were labeled with CFSE and stimulated with allogeneic APCs from BDF1 mice for 4 days; levels of proinflammatory cytokines (IFN-γ, TNF-α) in CD8 + T cells were analyzed by flow cytometry ( A ). Percentages of CFSE lo IFN-γ + CD8 + and CFSE lo TNF-α + CD8 + T cells among gated H2K d– CD8 + T cells are shown ( B ). ( C and D ) CD8 + T cells isolated from 2C transgenic WT or PERK-cKO mice were labeled with CFSE and stimulated with SIY peptides (10 nM) and syngeneic B6 APCs (Ly5.1); levels of cytokines were analyzed similarly. ( E – G ) Lethally irradiated BDF1 mice (1,200 cGy) received TCD-BM cells (5 × 10 6 ) alone or along with P815-luc cells (5,000) with or without CD25-removed T cells (3 × 10 6 ) from 2C WT or PERK-cKO donors. Body weight loss ( E ) and GVHD scores ( F ) were monitored after BMT. Tumor growth in BDF1 recipients was monitored via BLI ( G ); n = 5 per BMT group. ( H ) CD8 + T cells from pMEL WT or PERK-cKO mice were stimulated with gp100 peptides and B6 APCs (Ly5.1) for 3 days; levels of IFN-γ and IL-17 in CD8 + T cells were analyzed. Percentages of IFN-γ + CD8 + and IL-17 + CD8 + T cells among gated CD45.1 – CD8 + T cells are shown. ( I and J ) Lethally irradiated BDF1 mice received TCD-BM cells (5 × 10 6 ) along with B16F10-luc cells with or without CD25-removed T cells (3 × 10 6 ) from pMEL WT or PERK-cKO donors; n = 3 for tumor only, n = 5–6 for WT or PERK-cKO ( I ). Survival ( J ) was monitored after BMT. Log-rank (Mantel-Cox) test ( J ) and non-parametric Mann-Whitney U test ( E and F ) were used to compare groups. Data in B , D , and H are represented as mean ± SD with biological replicates; significance was determined using a 2-tailed unpaired Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: The Journal of Clinical Investigation

    Article Title: ER stress sensor PERK promotes T cell pathogenicity in GVHD by regulating ER-associated degradation

    doi: 10.1172/JCI190958

    Figure Lengend Snippet: ( A and B ) CD8 + T cells isolated from 2C transgenic WT or PERK-cKO mice were labeled with CFSE and stimulated with allogeneic APCs from BDF1 mice for 4 days; levels of proinflammatory cytokines (IFN-γ, TNF-α) in CD8 + T cells were analyzed by flow cytometry ( A ). Percentages of CFSE lo IFN-γ + CD8 + and CFSE lo TNF-α + CD8 + T cells among gated H2K d– CD8 + T cells are shown ( B ). ( C and D ) CD8 + T cells isolated from 2C transgenic WT or PERK-cKO mice were labeled with CFSE and stimulated with SIY peptides (10 nM) and syngeneic B6 APCs (Ly5.1); levels of cytokines were analyzed similarly. ( E – G ) Lethally irradiated BDF1 mice (1,200 cGy) received TCD-BM cells (5 × 10 6 ) alone or along with P815-luc cells (5,000) with or without CD25-removed T cells (3 × 10 6 ) from 2C WT or PERK-cKO donors. Body weight loss ( E ) and GVHD scores ( F ) were monitored after BMT. Tumor growth in BDF1 recipients was monitored via BLI ( G ); n = 5 per BMT group. ( H ) CD8 + T cells from pMEL WT or PERK-cKO mice were stimulated with gp100 peptides and B6 APCs (Ly5.1) for 3 days; levels of IFN-γ and IL-17 in CD8 + T cells were analyzed. Percentages of IFN-γ + CD8 + and IL-17 + CD8 + T cells among gated CD45.1 – CD8 + T cells are shown. ( I and J ) Lethally irradiated BDF1 mice received TCD-BM cells (5 × 10 6 ) along with B16F10-luc cells with or without CD25-removed T cells (3 × 10 6 ) from pMEL WT or PERK-cKO donors; n = 3 for tumor only, n = 5–6 for WT or PERK-cKO ( I ). Survival ( J ) was monitored after BMT. Log-rank (Mantel-Cox) test ( J ) and non-parametric Mann-Whitney U test ( E and F ) were used to compare groups. Data in B , D , and H are represented as mean ± SD with biological replicates; significance was determined using a 2-tailed unpaired Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: Luciferase-overexpressing B16F10 (B16F10-luc) cells were purchased from IVIS Imaging Co. Jurkat E6.1 cells were purchased from ATCC.

    Techniques: Isolation, Transgenic Assay, Labeling, Flow Cytometry, Irradiation, MANN-WHITNEY